In the recent decades, the use of pulsed-field gel electrophoresis (PFGE) in the molecular biology space has been subjected to a lot of research. Pulsed-field gel electrophoresis is a robust tool used to characterize various strains at the DNA level, to obtain useful information on genome size and to create the physical and genetic map of bacteria chromosome that are poorly understood at the genetic level. Pulsed-field gel electrophoresis is also used in isolating chromosomes in microorganisms, and in the long-range mapping of mammalian genes, and can also examine the extended and oriented configuration of huge DNA molecules in agarose gels at finite field strengths.
Genome analysis based on pulsed-field gel electrophoresis has a wide range of applications ranging from typing bacterial strains to radiobiology to cancer research, which could bring some traction in pulsed-field gel electrophoresis market. The methods for running pulsed-field gel electrophoresis have positively changed since its invention, but the method for preparing chromosomal DNA itself is essentially unchanged, which continues to restrict pulsed-field gel electrophoresis applicability, especially when analyses require many samples.
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Pulsed-field gel electrophoresis has been an integral part of food safety testing for long, and is still in use today, however it is quickly being replaced. For instance, FSIS (Food Safety and Inspection Service) has recently moved from PFGE to WGS (Whole Genome Sequencing) for analyzing Salmonella and Shiga Toxin-Producing E. coli (STEC) Isolates, as pulsed-field gel electrophoresis doesn’t differentiate isolates to the same degree that can be accomplished by WGS.
Pulsed Field Gel Electrophoresis (PFGE) Market: Drivers and Restraints
Primary factors driving Pulsed Field Gel Electrophoresis (PFGE) market include growing need for safe and effective guiding technology during isolation. PulseNet investigates bacterial isolates from sick people, contaminated food, and the places where food is produced. PFGE is the said to be the gold standard fingerprinting method used within PulseNet. However, some limitations of PFGE hindering the growth of pulsed field gel electrophoresis market.
Time consuming process, inability to discriminate between all unrelated isolates, variation in DNA restriction patterns, and inability to optimize separation in every part of the gel at the same time, are the main limiting factors which are restricting the growth of the market. Besides, bands of same size may not come from the same part of the chromosome, change in one restriction site can result in more than one band change, and inability to differentiate isolates to the same degree that can be achieved by whole genome sequencing (WGS) are other key restraining factors.
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Pulsed Field Gel Electrophoresis (PFGE) Market: Segmentation
Tentatively, the global Pulsed Field Gel Electrophoresis (PFGE) market has been segmented on the basis of indication, end users, and geography.
Based on Types, the global Pulsed Field Gel Electrophoresis (PFGE) market is segmented as:
- Rotating Gel Electrophoresis (RGE)
- Field-Inversion Gel Electrophoresis (FIGE)
- Transverse-Alternating Field Gel Electrophoresis (TAFE)
- Contour-Clamped Homogeneous Electric Fields (CHEF)
- Orthogonal-Field Alternation Gel Electrophoresis (OFAGE)
- Programmable Autonomously-Controlled Electrodes (PACE)
- Pulsed-Homogeneous Orthogonal Field Gel Electrophoresis (PHOGE)
Based on applications, the global Pulsed Field Gel Electrophoresis (PFGE) market is segmented as:
- Separation of chromosomes
- Restriction mapping of chromosome regions
- DNA fragment purification
- Others
Based on end user, the global Pulsed Field Gel Electrophoresis (PFGE) market is segmented as:
- Laboratory
- Academia
- Private Laboratories
- Biopharmaceuticals Companies
- CMOs
- Others
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Pulsed Field Gel Electrophoresis (PFGE) Market: Key Players
The global pulsed field gel electrophoresis systems market is consolidated market and dominated by the are some leading players such as Bio-Rad Laboratories, Inc, Core Life Sciences, Inc., LABREPCO, Analytik Jena AG. Technological advancement in products and expansion of geographical reach are the major trend observed in the global pulsed field gel electrophoresis systems market.
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