A thermal cycler or a DNA amplification machine performs polymerase chain reactions to make copies of genetic material and is popularly known as a PCR. The machine raises and lowers temperature at a highly sensitive pre-programmed steps in order to denature and renature DNA that is inserted within small tubes of the machine. The amplification of DNA is a precursor in experimentation and research in genomics and proteomics to study drug interactions, genome sequencing, protein sequencing and gene identification. PCR was introduced a few decades ago and have undergone revolutionary changes in terms of quality and speed. Although the working mechanism for a PCR is simple, their cost is primarily high due to the presence of highly sensitive temperature control system. Peltier and silver elements are primarily used in thermal cyclers to achieve temperature control in a PCR instrument. The term instant PCR signifies instant evaluation of the absolute or relative quantification of the cDNA copies from a single real time PCR curve. Instant PCR enables amplification of DNA strand in a short time period for formation of a number of identical DNA copies.
Some of the major market players operating in the global instant PCR instrument market includes:
Agilent Technologies, ThermoFisher Scientific, Life Technologies, Danaher Corporation, Sigma Aldrich, Perkin Elmer, Affymetrix, Illumina, Bruker-Daltonics among others.
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Due to the extensive genomics research throughout the world, instant PCR’s are very much in demand and account for a part of basic capital investment by research institutions in research equipment. Cancer remains unconquered; it is estimated that around 12 million people globally are diagnosed with cancer every year. There is an increase in the number of cancer cases due to the growing global elderly population and unhealthy lifestyle. Breast, lung, gastric and prostate cancers are the most frequently diagnosed type of cancer in industrialized countries. Instant PCR is mostly utilized for the diagnosis of cancer. With rising prevalence of various cancer the global demand of instant PCR instrument is also gaining popularity worldwide and thereby increases its demand. For instance, the World Health Organization (WHO) published in 2014, that worldwide 8.2 million people died of cancer in 2012.
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